While transcription-translation (TXTL) systems are powerful tools for screening recombinant proteins and antibodies, they frequently involve time-consuming bottlenecks during the synthesis of DNA templates. Although linear double-stranded DNA templates accelerate workflows, many commercial suppliers have struggled to provide the necessary length, complexity, or accuracy required to effectively replace plasmid- or PCR-based approaches. There is a need for more efficient and reliable DNA template solutions to fully leverage the advantages of TXTL systems in applications such as protein screening. Elegen’s ENFINIA™ Linear DNA improves TXTL screening by successfully addressing several bottlenecks associated with traditional DNA template synthesis.
In this GEN webinar, our expert speakers will present a case study demonstrating the exceptional properties and high-fidelity performance of ENFINIA Linear DNA as evaluated in GeneFrontier’s cell-free protein synthesis PUREfrex® system. These results showcase ENFINIA’s outperformance in terms of DNA purity, transcription efficiency, and robust protein expression for various constructs. Attendees will learn:
● How this powerful combination eliminates the need for cloning or PCR amplification, significantly streamlining TXTL workflows
● How to reduce hands-on time and enable higher-throughput protein screening for synthetic biology, protein engineering, and drug discovery applications
● How to apply ENFINIA Linear DNA for challenging sequences such as long repeats, GC-rich promoters, and homopolymers
A live Q&A session will follow the presentations, offering you a chance to pose questions to our expert panelists.
Produced with support from:
